Acute toxic liver injury. nicotinamide-adenine dinucleotide-pyrophosphorylase activity of nuclei isolated from rat liver in heliotrine and in dimethylnitrosamine poisoning
نویسندگان
چکیده
منابع مشابه
Acute toxic liver injury. nicotinamide-adenine dinucleotide-pyrophosphorylase activity of nuclei isolated from rat liver in heliotrine and in dimethylnitrosamine poisoning.
2. The nunmber of reactive SH groups/molecule in the haemoglobins studied approximate either to 2 or to 4. 3. The behaviour of the reactive SH groups differs from that of cysteine in three ways: (a) the rate of reaction of heavy-metal ions with the reactive SH groups is slower than with cysteine; (b) thiosulphate interferes with the reaction between Ag+ ions and the reactive SH groups of human ...
متن کاملNicotinamide Adenine Dinucleotide Glycohydrolase from Rat Liver Nuclei
A new type of nicotinamide adenine dinucleotide glycohydrolase (NADase) has been isolated from rat liver nuclei. When partially purified chromatin is passed through a Sephadex G-200 column in the presence of 1 M NaCl, enzyme activities catalyzing the liberation of nicotinamide from NAD elute in two peaks. One, which appears in the void volume fraction, hydrolyzes the nicotinamide-ribose linkage...
متن کاملInhibition of rat liver nicotinamide adenine dinucleotide kinase by reduced nicotinamide adenine dinucleotide phosphate.
Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...
متن کاملNicotinamide adenine dinucleotide glycohydrolase from rat liver nuclei. Isolation and characterization of a new enzyme.
A new type of nicotinamide adenine dinucleotide glycohydrolase (NADase) has been isolated from rat liver nuclei. When partially purified chromatin is passed through a Sephadex G-200 column in the presence of 1 M NaCl, enzyme activities catalyzing the liberation of nicotinamide from NAD elute in two peaks. One, which appears in the void volume fraction, hydrolyzes the nicotinamide-ribose linkage...
متن کاملLiver Damage in Acute Heliotrine Poisoning 2. THE EFFECT OF NICOTINAMIDE ON PYRIDINE NUCLEOTIDE
Earlier studies on the effects of heliotrine poisoning demonstrated an apparent deficiency of DPN in homogenate and mitochondrial preparations from the livers of heliotrine-poisoned (1620hr.) rats (Christie, 1958b). More recent work has shown that the concentrations of pyridine nucleotides were, in fact, decreased in homogenates and in the mitochondrial and soluble fractions of liver from rats ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1962
ISSN: 0006-2936
DOI: 10.1042/bj0840364